Western blot shows lysates of HT1080 human fibrosarcoma cell line. PVDF membrane was probed with 1ug/ml of Sheep Mouse Collagen XIII a1 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Sheep IgG Secondary Antibody. A specific band was detected for Collagen XIII a1 at approximately 85-95kD. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CollagenXIII a1 in Mouse Embryo. Collagen XIII a1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti -Mouse Collagen XIII a1 Antigen Affinity-purified Polyclonal Antibody at 15ug/ml overnight at 4°C. Tissue was stained using the Sheep HRP-DAB Cell & Tissue Staining Kit aNone Detected counterstained with hematoxylin (blue). Specific staining was localized to spinal cord.
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